Ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) was synthesized
by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials.
The hydrodynamic diameter of PIPAAm-ferritins in aqueous solution increased at 37 ◦C at a higher protein
concentration (>0.2 mg/mL) because of the intermolecular aggregation through the hydrophobic interaction
of PIPAAm chains. On the other hand, PIPAAm-ferritins at a lower concentration (<0.2 mg/mL) were
unable to increase their size even at 37 ◦C. The adsorption kinetics of PIPAAm-ferritins on hydrophobically
modified Si substrate were evaluated with a quartz crystal microbalance in 10 mmol/L Bis–Tris/HCl buffer
(pH 5.8) with and without poly(oxyethylene) sorbitan monolaurate (TWEEN 20) (0.05 wt%) as a surfactant.
Although the adsorption of PIPAAm-ferritins on hydrophobic Si substrate at 25 ◦C in the buffer with
TWEEN 20 was hardly observed, PIPAAm-ferritins were considerably adsorbed on the substrate at 37 ◦C,
indicating that the hydrophobic interaction between the substrate and PIPAAm grafts on the ferritins
after the destruction of the hydrophobic interaction between the protein and the substrate by TWEEN
20.
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