Methane fermn. of the syrup wastewater was conducted using the anaerobic sequencing batch reactor (ASBR). Org. loading rate (OLR), which is calcd. by dividing the substrate concn. in the wastewater by hydraulic retention time (HRT), was set at the same value in two runs; R1 with longer HRT and higher concn. of substrate, and R2 with shorter HRT and lower concn. of substrate. The effects of HRT and substrate concn. on the performance of the methane fermn. were tried to be elucidated by comparing two exptl. runs. The OLR was increased stepwisely from 2.6 to 20.4 g-COD/L/d. No significant difference was obsd. until the OLR of 10.2 g-COD/L/d. After the OLR increased to 20.4 g-COD/L/d, oxidn. redn. potential (ORP) in R2 with short HRT of 3.1 days increased immediately and methane gas prodn. rate (MPR) decreased drastically. In contrast, the ORP of R1 with long HRT of 6.2 days was in the range from -450 to -350 mV, and high MPR was maintained for a while though the OLR in R1 was the same with that in R2. More than 10 days of operation with the OLR of 20.4 g-COD/L/d, however, the pH in R1 decreased abruptly and the MPR also decreased rapidly. It was revealed that the collapse of methane fermn. caused by the too short HRT occurred faster than that caused by the too high concn. of substrate.
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