Deoxyribozymes (DNAzymes) having RNA-cleaving activity have widely been explored as tools for
therapeutic and diagnostic purposes. Both the chemical cleaving step and the turnover step should
be improved for enhancing overall activity of DNAzymes. We have shown that cationic copolymer
enhanced DNAzyme activity by increasing turnover efficacy. In this paper, effects of the copolymer
on DNAzymes modified with locked nucleic acids (LNA) or 2′-O-methylated (2′-OMe) nucleic acids
were studied. The copolymer increased activity of these chemically modified DNAzymes. More than
30-fold enhancement in multiple-turnover catalytic activity was observed with 2′-OMe-modified
DNAzyme in the presence of the copolymer. DNAzyme catalytic activity was successfully enhanced
by cooperation of the added copolymer and chemical modification of DNAzyme.
各種検索
サポート
ヘルプ