Single-mol. fluorescence resonance energy transfer (smFRET) is a powerful tool to study the dynamics of biomol. events in real time. However, it requires two fluorophores and can be applied only to dynamics that accompany large changes in distance between the mols. Herein, the authors introduce a method for kinetic anal. based on control of fluorescence blinking (KACB), a general approach to study the dynamics of biomols. by using a single fluorophore. By controlling the kinetics of the redox reaction the blinking kinetics or pattern can be controlled to be affected by microenvironmental changes around a fluorophore (rKACB), thereby enabling real-time single-mol. measurement of the structure-changing dynamics of nucleic acids.
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