論文

• Tadashi Wada, Masatoshi Hara, Takuya Taneda, Cao Qingfu, Ryouhei Takata, Kanako Moro, Kei Takeda, Takeo Kishimoto, Hiroshi Handa. Antisense morpholino targeting just upstream from a poly(A) tail junction of maternal mRNA removes the tail and inhibits translation, Nucleic Acids Res., Vol. 40, No. 22, p. e173, Dec. 2012.
• Takuya Taneda, Wenyan Zhu, Qingfu Cao, Hajime Watanabe, Yuki Yamaguchi, Hiroshi Handa, Tadashi Wada. Erythropoiesis is regulated by the transcription elongation factor Foggy/Spt5 through gata1 gene regulation, Genes to Cells, 16, 2, 231–242, Feb. 2011.
• Sol Kim, Junichi Yamamoto, Yexi Chen, Masatoshi Aida, Tadashi Wada, Hiroshi Handa, Yuki Yamaguchi. Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation, Genes Cells, Vol. 15, No. 9, p. 1003-1013, Sept. 2010.
• Kozue Ando, Satoshi Hirao, Yasuaki Kabe, Yuji Ogura, Iwao Sato, Yuki Yamaguchi, Tadashi Wada, Hiroshi Handa. A new APE1/Ref-1-dependent pathway leading to reduction of NF-kappaB and AP-1, and activation of their DNA-binding activity, Nucleic Acids Res., 36, 13, 4327-4336, Aug. 2008.
• Yugami M, Kabe Y, Yamaguchi Y, Wada T, Handa. hnRNP-U enhances the expression of specific genes by stabilizing mRNA., FEBS Letters, Vol. 581, pp. 1-7, Jan. 2007.
• Ogura Y, Azuma M, Tsuboi Y, Kabe Y, Yamaguchi Y, Wada T, Watanabe H, Handa H. TFII-I down-regulates a subset of estrogen-responsive genes through its interaction with an initiator element and estrogen receptor alpha., Genes Cells, Vol. 11, pp. 373-381, 2006.
• Kawano MA, Inoue T, Tsukamoto H, Takaya T, Enomoto T, Takahashi RU, Yokoyama N, Yamamoto N, Nakanishi A, Imai T, Wada T, Kataoka K, Handa H. The VP2/VP3 minor capsid protein of simian virus 40 promotes the in vitro assembly of the major capsid protein VP1 into particles., J Biol Chem, Vol. 281, pp. 10164-10173, 2006.
• Aida M, Chen Y, Nakajima K, Yamaguchi Y, Wada T, Handa H. Transcriptional pausing caused by NELF plays a dual role in regulating immediate-early expression of the junB gene., Mol Cell Biol, Vol. 26, pp. 6094-6104, 2006.
• Kubo T, Wada T, Yamaguchi Y, Shimizu A, Handa H. Knock-down of 25 kDa subunit of cleavage factor Im in HeLa cells alters alternative polyadenylation within 3’-UTRs., Nucleic Acid Res, Vol. 34, pp. 6264-6271, 2006.
• Urano A, Endoh M, Wada T, Morikawa Y, Itoh M, Kataoka Y, Taki T, Akazawa H, Nakajima H, Komuro I, Yoshida I, Hayashi Y, Handa H, Kitamura T, Nosaka T. Infertility with defective spermiogenesis in mice lacking AF5q31, the target of chromosomal translocation in human infant leukemia., Mol Cell Biol, Vol. 25, pp. 6834-6845, 2005.
• SS. Mandal, C.Chu, T.Wada, H. Handa, AJ.Shatkin, D.Reinberg.. Functional interactions of RNA-capping enzyme with factors that positively and negatively regulate promoter escape by RNA polymerase II., Proc Natl Acad Sci U S A, Vol. 101, pp. 7572-7577, 2004.
• C. Sawa, E. Nedea, N. Krigan, T.Wada, H.Handa, J. Greenblatt, S. Buratowski.. Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2., Mol Cell Biol, Vol. 24, pp. 4734-4742, 2004.
• Hasegawa J, Endou M, Narita T, Yamada T, Yamaguchi Y, Wada T, Handa H. A rapid purification method for human RNA polymerase II by two-step affinity chromatography., J Biochem, Vol. 133, pp. 133-138, 2003.
• Kuraoka I, Endou M, Yamaguchi Y, Wada T, Handa H, Tanaka K. Effects of endogenous DNA base lesions on transcription elongation by mammalian RNA polymerase II. Implications for transcription-coupled DNA repair and transcriptional mutagenesis., J Biol Chem, Vol. 278, pp. 7294-7299, 2003.
• Ohbayashi T, Shimada M, Nakadai T, Wada T, Handa H, Tamura T. Vertebrate TBP-like protein (TLP/TRF2/TLF) stimulates TATA-less terminal deoxynucleotidyl transferase promoters in a transient reporter assay, and TFIIA-binding capacity of TLP is required for this function. , Nucleic Acids Res, Vol. 31, pp. 2127-2133, 2003.
• Narita T, Yamaguchi Y, Yano K, Sugimoto K, Chanarat S, Wada T, Kim DK, Hasegawa J, Omori M, Inukai N, Endoh M, Yamada T, Handa H. Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex., Mol Cell Biol, Vol. 23, pp. 1863-1873, 2003.
• Shima D, Yugami M, Tatsuno M, Wada T, Yamaguchi Y, Handa H. Mechanism of H-8 inhibition of Cyclin-dependent kinase 9: study using inhibitor-immobilized matrices. , Genes Cells, Vol. 8, 215-223,, 2003.
• Kim DK, Inukai N, Yamada T, Furuya A, Sato H, Yamaguchi Y, Wada T, Handa H. Structure-function analysis of human Spt4: evidence that hSpt4 and hSpt5 exert their roles in transcriptional elongation as parts of the DSIF complex. , Genes Cells, Vol. 8, pp. 371-378, 2003.
• Y.Yamaguchi, N.Inukai, T.Narita, T.Wada, H.Handa.. Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA., Mol. Cell. Biol., Vol. 22, pp. 2918-2927, 2002.
• Y.Yamaguchi, J.Filipovska, K.Yano, A.Furuya, N.Inukai, T.Narita, T.Wada, S. Sugimoto, M.M.Konarska, H. Handa.. Stimulation of RNA polymerase II elongation by Hepatitis Delta Antigen., Science, Vol. 293, No. 5527, pp. 124-127, 2001.
• D.B. Renner, Y.Yamaguchi, T.Wada, H.Handa, D.H.Price.. A highly purified RNA polymerase II elongation control system., J. Biol. Chem., Vol. 276, pp. 42601-42609, 2001.
• Y.Yamaguchi, T.Narita, N.Inukai, T.Wada, H.Handa.. SPT genes: Key players in the regulation of transcription, chromatin structure and other cellular processes (review)., J. Biochemistry, Vol. 129, pp. 185-191, 2001.
• D.Kim, Y.Yamaguchi, T.Wada, H.Handa.. The Regulation of Elongation by Eukaryotic RNA Polymerase II: A Recent View., Molecules and Cells, Vol. 11, pp. 267-274, 2001.
• S.Guo, Y.Yamaguchi, S.Schilbach, T.Wada, J.Lee, A.Goddard, D.French, H.Handa, A.Rosenthal.. A regulator of transcriptional elongation controls vertebrate neuronal development., Nature, Vol. 408, No. 6810, pp. 366-369, 2000.
• T.Wada, G.Orphanides, J.Hasegawa, D.Kim, D.Shima, Y.Yamaguchi, A.Fukuda, K.Hisatake, S.Oh, D.Reinberg, H.Handa.. FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH., Molecular Cell, Vol. 5, No. 6, pp. 1067-1072, 2000.
• H. Tsutsui, C. Geltinger, T. Murata, K. Itakura, T. Wada, H. Handa, K. K. Yokoyama.. The DNA-binding and transcriptional activities of MAZ, a Myc-associated zinc finger protein, are regulated by casein kinase II., Biochem. Biophys. Res. Commun., Vol. 262, pp. 198-205, 1999.
• H. Handa, T. Takagi, T. Wada, J. Hasegawa, Y. Yamaguchi.. Regulatory network of positive and negative elongation facotrs:interplay between DSIF, NELF and P-TEFb., Mechanisms of Eukaryotic Transcription, 1999.
• Y. Kabe, M. Goto, D. Shima, T. Imai, T. Wada, et al.. The Role of Human MBF1 as a Transcriptional Coactivator., J. Biol. Chem., Vol. 274, pp. 34196-34202, 1999.
• Y. Yamaguchi, T. Takagi, T. Wada, K. Yano, A. Furuya, S. Sugimoto, J. Hasegawa, H. Handa.. NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation., Cell, Vol. 97, pp. 41-51, 1999.
• J.B. Kim, Y. Yamaguchi, T. Wada, H. Handa, P.A. Sharp.. Tat-SF1 associates with Rap30 and hSpt5 proteins., Mol. Cell. Biol., Vol. 19, pp. 5960-5968, 1999.
• Y. Yamaguchi, T. Wada, D. Watanabe, T. Takagi, J. Hasegawa, H. Handa.. Structure and function of the human transcription elongation factor DSIF., J. Biol. Chem., Vol. 274, pp. 8085-8092, 1999.
• TADASHI WADA. Human Spt4 and Spt5 homologs regulates RNA polymerase II elongation and show structual and functional similarities to the E. coli elongation factor Nus G., The Fifth Asian Conference on Transcription, Lorne(Australia), 1998.
• Y. Yamaguchi, T. Wada, F. Suzuki, T. Takagi, J. Hasegawa, H. Handa.. Casein kinase II interacts with bZIP domains of several transcription factors., Nucleic Acids Res., Vol. 26, pp. 3854-3861, 1998.
• T. Wada, T. Takagi, Y. Yamaguchi, D. Watanabe, H. Handa.. Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro., EMBO J., Vol. 17, pp. 7395-7403, 1998.
• Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H. DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs., Genes & Development, Vol. 12, pp. 343-356, 1998.
• Y. Yamaguchi, T. Wada, H. Handa.. Interplay between positive and negative elongation factors : drawing a new view of DRB., Genes to Cells, Vol. 3, pp. 9-15, 1998.
• TADASHI WADA. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae., Genes & Development, Vol. 12, pp. 357-369, 1998.
• 和田忠士. DRBによる転写抑制機構-カゼインキナーゼIIによる転写制御の可能性, 実験医学, Vol. 15, No. 4, pp. 44, 1997.
• TADASHI WADA. Phosphorylation and high level expression of Fra-2 in v-srctransformed cells : a pathway of activation of endogenous AP-1., Oncogene, Vol. 14, pp. 2435, 1997.
• TADASHI WADA. DSIF, a novel negative transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs, Mechanism of Eukaryotic Transcription Cold Spring Harbor Laforatory(USA)., 1997.
• TADASHI WADA. Regulation of RNA polymerase II processivity by casein kinase II., EMBL Meeting on Transcription(Heidelberg), 1996.
• 和田忠士. DNA腫瘍ウイルスの分子生物学の現状, 蛋白質核酸酸素, Vol. 41, No. 6, pp. 793, 1996.
• TADASHI WADA. A transcription factor with leucine-zipper motif involved in light-dependent inhibition of expression of the puf aporon in the photosynthetic facterium Rhodofacter sphaeroides., Plant Cell Physiol., Vol. 37, pp. 515-522, 1996.
• TADASHI WADA. Involvement of casein kinase II activity in transcription initiation., The Fourth Asian Conference on Transcription(Hayama), 1996.
• TADASHI WADA. Copuritication of Casein Kinase II with transcription Factor ATF/E4TF3, Nucleic Acids Res., Vol. 24, pp. 876, 1996.
• TADASHI WADA. Purification of transcription factor IIA using yeast TATA-binding protein affinity latex particles, Inter. J. Bio-Chromato., 1995.
• TADASHI WADA. Cloning a nuclear binding factor (NF2d9) to the male-specific p450 (Cyp2d-9) gene in mouse liver, Mole. Cell. Biol., 1995.
• TADASHI WADA. DNA Affinity Chromatography, Methoda in Enzymology, Vol. 254, pp. 595, 1995.
• 和田忠士. DNA腫瘍ウイルスの分子生物学の現状, 蛋白質核酸酵素, Vol. 40, pp. 932-939, 1995.
• TADASHI WADA. Construction of a cDNA lefrarey for a specific region of a chromosome using a novel cDNA selection method utilizing latex particles., Gene, Vol. 165, pp. 155-161, 1995.
• TADASHI WADA. Preparation of DNA-carrying affinity latex and purification of transcription factors with the latex, J. Biomater. Sci. Polymer Edn., Vol. 5, pp. 293-302, 1994.
• TADASHI WADA. Identification of ATF1 associated protein kinase, AAK1, is a member of the casein kinase II family, DNA Tumor Virus Meeting, Cold Spring Habor Laboratory (USA), 1994.
• TADASHI WADA. Application of cationic latex particles for protein separation, Colloids and Surfaces, Vol. 2, pp. 419-427, 1994.
• TADASHI WADA. Purification and Characterization of protein kinases associated with transcription factor ATF/E4TF3 family, Asian Conference on Transcription (ACT III), Bangalore, (India), 1994.
• 和田忠士. DNA腫瘍ウイルスの分子生物学の現状, 蛋白質核酸酵素, Vol. 39, pp. 252-261, 1994.
• 和田忠士. DNAウイルス(転写調節モデル系として), Mebio, Vol. 11, pp. 50-57, 1994.
• TADASHI WADA. Purification and characterization of protein kinases associated with transcription factor E4TF3/ATF family, DNA Tumor Virus Meeting, Cambridge (UK), 1993.
• 和田忠士. SV40,ポリオーマウイルス,アデノウイルスの分子生物学-最近の動向 II, 蛋白質核酸酵素, Vol. 38, pp. 1402-1410, 1993.
• TADASHI WADA. Isolation of a cDNA encoding the largest subunits of TFIIA reveals functions similar to coactivators, Genes & Dev., Vol. 7, pp. 2246-2257, 1993.
• 和田忠士. アデノウイルスの転写調節, 生物物理, Vol. 32, pp. 66-70, 1992.
• 和田忠士. アデノウイルスの複製と遺伝子発現, 蛋白質核酸酵素, Vol. 37, pp. 2511-2518, 1992.
• TADASHI WADA. Direct purification of multiple ATF/E4TF3 polypeptides from HeLa cell crude nuclear extracts using DNA affinity latex particles, Analytical Biochem., Vol. 206, pp. 109-114, 1992.
• TADASHI WADA. Cooperative transcription activation between Ad1, CRE-like element and other elements in CYP11B, J. Biochem., Vol. 112, pp. 573-575, 1992.
• 和田忠士. SV40,ポリオーマウイルス,アデノウイルスの分子生物学-最近の動向, 蛋白質核酸酵素, Vol. 36, pp. 910-918, 1991.
• TADASHI WADA. E1A gene products stimulate in vitro transcription from the Adenovirus early region 4 promoter by enhancing a stable preinitiation complex, Virus Genes, Vol. 5, pp. 47-55, 1991.
• TADASHI WADA. Different biological activities of the hetero- and homodimers formed by the 47- and 43-kilodalton proteins of transcription factor ATF/E4TF3, J. Virol., Vol. 65, pp. 557-564, 1991.
• 和田忠士. DNAアフィニティークロマトグラフィーラテックス粒子による転写因子の精製, Cell Science, Vol. 7, pp. 5-13, 1991.
• 和田忠士. 転写調節における蛋白質因子間相互作用-特にアデノウイルスE4遺伝子の場合-, 蛋白質核酸酵素, Vol. 35, pp. 2825-2831, 1990.
• TADASHI WADA. Different biological activities of the hetero- and homodimers formed by the 47kD and 43kD proteins of transcription factor ATF/E4TF3, DNA Tumor Virus Meeting, Cold Spring Habor Laboratory (USA), 1990.
• TADASHI WADA. Strain-specific lethal effect of the adenovirus Ela protein on ┣DBSaccaromyces cerevisiae(/)-┫DB, Biochem. Biophys. Res. Comm., Vol. 170, pp. 470-476, 1990.
• TADASHI WADA. Transcription factor E4TF1 contains two subunits with different functions, EMBO, J., Vol. 9, pp. 841-847, 1990.
• TADASHI WADA. Purification of transcription factors by using latex particles, DNA Tumor Virus Meeting, Cambridge, 1989.
• TADASHI WADA. Purification of DNA-binding transcription factors by their selective adsorption on the affinity latex particles, Nucleic Acid Res, Vol. 17, pp. 6229-6240, 1989.
• TADASHI WADA. Efficient usage of a galactose-inducible expression vector for the production of heterologous protein in yeast, Agric. Biol. Chem., Vol. 52, pp. 2035-2041, 1988.
• TADASHI WADA. Nature of growth inhibition induced by expression of human adenovirus E1a protein in Saccharomyces cerevisiae, Yeast Genetics and Molecular Biology Meeting, San Francisco, 1987.
• TADASHI WADA. Expression of the human adenovirus Ela product in yeast, Gene, Vol. 58, pp. 127-136, 1987.

著書

• 半田宏, 和田忠士, 坂本聡. 診断学、１．ＭＥＭＳと診断 他, ＭＥＭＳ／ＮＥＭＳ工学全集, 株式会社テクノシステム, p. 212-217, 2009.
• 和田忠士, 山口雄輝, 半田宏. 転写研究集中マスター, 羊土社, pp. 12-26, 2006.
• 相田将俊, 和田忠士. 基本転写装置を調節するヒストン修飾, 細胞工学, 細胞工学, Vol. 23, No. 10, pp. 1139-1143, 2004.
• 和田忠士, 山口雄輝, 半田宏. mRNA合成速度の分子スイッチ, 蛋白質核酸酵素, 蛋白質核酸酵素, Vol. 48, No. 1, pp. 9-17, 2003.
• 和田忠士, 半田宏. 転写とクロマチン構造, シュプリンガー・フェアラーク東京, シュプリンガー・フェアラーク東京, pp. 165-175, 2003.
• 和田忠士. クロマチン環境下での転写開始と伸長反応にかかわる因子群, 実験医学, 実験医学, Vol. 10, No. 19-16, pp. 2165-2170, 2001.
• 和田忠士, 山口雄輝, 嶋大輔, 半田宏. RNAポリメラーゼIIの転写伸長反応制御, シュプリンガー・フェアラーク東京, シュプリンガー・フェアラーク東京, 2000.
• H. Handa, Y. Yamaguchi, T. Wada.. Purification of DNA-binding proteins. High Resolution Chromatography., A Pratical Approach edited by Paul Millner (IRL PRESS), A Pratical Approach edited by Paul Millner (IRL PRESS), pp. 283-301, 1999.
• 和田忠士. 基本転写装置の活性制御, 転写のメカニズムと疾患, 転写のメカニズムと疾患, pp. 30-35, 1997.

学位論文

• 転写因子E4TF3のホモおよびヘテロ２量体の生化学的解析,  本文,  Doctor of Medicine,  東京大学大学院医学系研究科第三基礎医学専攻,  1992/--/--, 

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