@book{CTT100704122, author = {Nobutaka Nakashima and Goh, S. and Good, L. and Tamura, T.}, title = {Multiple-Gene Silencing Using Antisense RNAs in Escherichia coli}, publisher = {}, year = 2012, } @article{CTT100806827, author = {Kentaro Akiyama and Kazuki Fujisawa and Hiro Kondo and Yuya Netsu and KojiNishikawa and Yoshio Takata and Yuya Nakamura and Yuta Kino and Shotaro Ayukawa and Masayuki Yamamura and NobuhiroHayashi and Yoh-ichi Tagawa and Nobutaka Nakashima}, title = {MazF activation causes ACA sequence-independent and selective alterations in RNA levels in Escherichia coli}, journal = {Springer Nature}, year = 2019, } @article{CTT100704117, author = {Akita, Hironaga and Nobutaka Nakashima and Hoshino, Tamotsu}, title = {Bacterial production of isobutanol without expensive reagents}, journal = {Applied Microbiology and Biotechnology}, year = 2015, } @article{CTT100704114, author = {Akita, H. and Watanabe, M. and Suzuki, T. and Nakashima, N. and Hoshino, T. and Nobutaka Nakashima}, title = {Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1}, journal = {FEMS Microbiology Letters}, year = 2015, } @article{CTT100704115, author = {Tsukuda, M. and Nobutaka Nakashima and Miyazaki, K.}, title = {Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli}, journal = {Journal of Bioscience and Bioengineering}, year = 2015, } @article{CTT100704116, author = {Akita, H. and Watanabe, M. and Suzuki, T. and Nobutaka Nakashima and Hoshino, T.}, title = {Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase}, journal = {AMB Express}, year = 2015, } @article{CTT100704120, author = {Nobutaka Nakashima and Ohno, S. and Yoshikawa, K. and Shimizu, H. and Tamura, T.}, title = {A vector library for silencing central carbon metabolism genes with antisense RNAs in escherichia coli}, journal = {Applied and Environmental Microbiology}, year = 2014, } @article{CTT100704118, author = {Nobutaka Nakashima and Akita, H. and Hoshino, T.}, title = {Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin}, journal = {Metabolic Engineering}, year = 2014, } @article{CTT100704119, author = {Nobutaka Nakashima and Miyazaki, K.}, title = {Bacterial cellular engineering by genome editing and gene silencing}, journal = {International Journal of Molecular Sciences}, year = 2014, } @article{CTT100704121, author = {Nobutaka Nakashima and Tamura, T.}, title = {Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline-inducible vectors}, journal = {Letters in Applied Microbiology}, year = 2013, } @article{CTT100704112, author = {Nobutaka Nakashima}, title = {Subtracting gene function by gene silencing and disruption in bacteria.}, journal = {}, year = 2012, } @article{CTT100704123, author = {Kagawa, Y. and Mitani, Y. and Yun, H.-Y. and Nobutaka Nakashima and Tamura, N. and Tamura, T.}, title = {Identification of a methanol-inducible promoter from Rhodococcus erythropolis PR4 and its use as an expression vector}, journal = {Journal of Bioscience and Bioengineering}, year = 2012, } @article{CTT100704124, author = {Nobutaka Nakashima and Tamura, T.}, title = {A new carbon catabolite repression mutation of Escherichia coli, mlc, and its use for producing isobutanol}, journal = {Journal of Bioscience and Bioengineering}, year = 2012, } @article{CTT100704125, author = {Nobutaka Nakashima and Tamura, T.}, title = {Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli}, journal = {Nucleic Acids Research}, year = 2009, } @article{CTT100704126, author = {Goh, S. and Boberek, J.M. and Nobutaka Nakashima and Stach, J. and Good, L.}, title = {Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli}, journal = {PLoS ONE}, year = 2009, } @article{CTT100704113, author = {Nobutaka Nakashima}, title = {Advances in the development of genetic tools for the genus Rhodococcus}, journal = {}, year = 2006, } @article{CTT100704129, author = {Kimura, N. and Kitagawa, W. and Mori, T. and Nobutaka Nakashima and Tamura, T. and Kamagata, Y.}, title = {Genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from Rhodococcus opacus strain SAO101}, journal = {Applied Microbiology and Biotechnology}, year = 2006, } @article{CTT100704127, author = {Nobutaka Nakashima and Tamura, T. and Good, L.}, title = {Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli}, journal = {Nucleic Acids Research}, year = 2006, } @article{CTT100704131, author = {Todaka, Y. and Wang, Y. and Tashiro, K. and Nobutaka Nakashima and Nishimoto, T. and Sekiguchi, T.}, title = {Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae}, journal = {Genetics}, year = 2005, } @article{CTT100704132, author = {Nobutaka Nakashima and Mitani, Y. and Tamura, T.}, title = {Actinomycetes as host cells for production of recombinant proteins}, journal = {Microbial Cell Factories}, year = 2005, } @article{CTT100704130, author = {Wang, Y. and Nobutaka Nakashima and Sekiguchi, T. and Nishimoto, T.}, title = {Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p}, journal = {Biochemical and Biophysical Research Communications}, year = 2005, } @article{CTT100704134, author = {Nobutaka Nakashima and Tamura, T.}, title = {Isolation and characterization of a rolling-circle-type plasmid from Rhodococcus erythropolis and application of the plasmid to multiple-recombinant- protein expression}, journal = {Applied and Environmental Microbiology}, year = 2004, } @article{CTT100704135, author = {Nobutaka Nakashima and Tamura, T.}, title = {Cell-free protein synthesis using cell extract of Pseudomonas fluorescens and CspA promoter}, journal = {Biochemical and Biophysical Research Communications}, year = 2004, } @article{CTT100704136, author = {Nobutaka Nakashima and Tamura, T.}, title = {A Novel System for Expressing Recombinant Proteins over a Wide Temperature Range from 4 to 35°C}, journal = {Biotechnology and Bioengineering}, year = 2004, } @article{CTT100704137, author = {Sekiguchi, T. and Todaka, Y. and Wang, Y. and Hirose, E. and Nobutaka Nakashima and Nishimoto, T.}, title = {A Novel Human Nucleolar Protein, Nop132, Binds to the G Proteins, RRAG A/C/D}, journal = {Journal of Biological Chemistry}, year = 2004, } @article{CTT100704133, author = {Dong, L. and Nobutaka Nakashima and Tamura, N. and Tamura, T.}, title = {Isolation and characterization of the Rhodococcus opacus thiostrepton-inducible genes tipAL and tipAS: Application for recombinant protein expression in Rhodococcus}, journal = {FEMS Microbiology Letters}, year = 2004, } @article{CTT100704139, author = {Sekiguchi, T. and Hirose, E. and Nobutaka Nakashima and Ii, M. and Nishimoto, T.}, title = {Novel G Proteins, Rag C and Rag D, Interact with GTP-binding Proteins, Rag A and Rag B}, journal = {Journal of Biological Chemistry}, year = 2001, } @article{CTT100704138, author = {Suzuki, N. and Noguchi, E. and Nobutaka Nakashima and Oki, M. and Ohba, T. and Tartakoff, A. and Ohishi, M. and Nishimoto, T.}, title = {The Saccharomyces cerevisiae small GTPase, gsp1p/Ran, is involved in 3′ processing of 7S-to-5.8S rRNA and in degradation of the excised 5′-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome}, journal = {Genetics}, year = 2001, } @article{CTT100704140, author = {Nobutaka Nakashima and Noguchi, E. and Nishimoto, T.}, title = {Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p}, journal = {Genetics}, year = 1999, } @article{CTT100704142, author = {Shiomi, T. and Fukushima, K. and Suzuki, N. and Nobutaka Nakashima and Noguchi, E. and Nishimoto, T.}, title = {Human Dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3}, journal = {Journal of Biochemistry}, year = 1998, } @article{CTT100704143, author = {Shiomi, T. and Fukushima, K. and Suzuki, N. and Nobutaka Nakashima and Noguchi, E. and Nishimoto, T.}, title = {Correction: Human Dis3p, which binds to either GTP- or GDP-Ran, complements saccharomyces cerevisiae dis3 (Journal of Biochemistry (1998) 123, 5 (883-890))}, journal = {Journal of Biochemistry}, year = 1998, } @article{CTT100704141, author = {Hirose, E. and Nobutaka Nakashima and Sekiguchi, T. and Nishimoto, T.}, title = {RagA is a functional homologue of S. cerevisiae Gtr1p involved in the Ran/Gsp1-GTPase pathway}, journal = {Journal of Cell Science}, year = 1998, } @article{CTT100704144, author = {Noguchi, E. and Hayashi, N. and Nobutaka Nakashima and Nishimoto, T.}, title = {Yrb2p, a Nup2p-related yeast protein, has a functional overlap with Rna1p, a yeast Ran-GTPase-activating protein}, journal = {Molecular and Cellular Biology}, year = 1997, } @article{CTT100704146, author = {Noguchi, E. and Hayashi, N. and Azuma, Y. and Seki, T. and Nakamura, M. and Nobutaka Nakashima and Yanagida, M. and He, X. and Mueller, U. and Sazer, S. and Nishimoto, T.}, title = {Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1}, journal = {EMBO Journal}, year = 1996, } @article{CTT100704145, author = {Nobutaka Nakashima and Hayashi, N. and Noguchi, E. and Nishimoto, T.}, title = {Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae}, journal = {Journal of Cell Science}, year = 1996, } @inproceedings{CTT100704128, author = {Nobutaka Nakashima and Tamura, T and Good, L}, title = {Large-loop antisense RNA hairpins provide stabilized and highly efficient antisense regulators in Escherichia coli}, booktitle = {Faseb Journal}, year = 2006, }