論文

• Kentaro Akiyama, Kazuki Fujisawa, Hiro Kondo, Yuya Netsu, KojiNishikawa, Yoshio Takata, Yuya Nakamura, Yuta Kino, Shotaro Ayukawa, Masayuki Yamamura, NobuhiroHayashi, Yoh-ichi Tagawa, Nobutaka Nakashima. MazF activation causes ACA sequence-independent and selective alterations in RNA levels in Escherichia coli, Springer Nature, Springer Nature, Sept. 2019.  公式リンク  
  
• Akita, Hironaga, Nobutaka Nakashima, Hoshino, Tamotsu. Bacterial production of isobutanol without expensive reagents, Applied Microbiology and Biotechnology, Vol. 99, No. 2, pp. 991-999, 2015.  公式リンク  
  
• Akita, H., Watanabe, M., Suzuki, T., Nakashima, N., Hoshino, T., Nobutaka Nakashima. Molecular cloning and characterization of two YGL039w genes encoding broad specificity NADPH-dependent aldehyde reductases from Kluyveromyces marxianus strain DMB1, FEMS Microbiology Letters, Vol. 362, No. 16, 2015.  公式リンク  
  
• Tsukuda, M., Nobutaka Nakashima, Miyazaki, K.. Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli, Journal of Bioscience and Bioengineering, Vol. 120, No. 5, pp. 591-595, 2015.  公式リンク  
  
• Akita, H., Watanabe, M., Suzuki, T., Nobutaka Nakashima, Hoshino, T.. Characterization of the Kluyveromyces marxianus strain DMB1 YGL157w gene product as a broad specificity NADPH-dependent aldehyde reductase, AMB Express, Vol. 5, No. 1, 2015.  公式リンク  
  
• Nobutaka Nakashima, Ohno, S., Yoshikawa, K., Shimizu, H., Tamura, T.. A vector library for silencing central carbon metabolism genes with antisense RNAs in escherichia coli, Applied and Environmental Microbiology, Vol. 80, No. 2, pp. 564-573, 2014.  公式リンク  
  
• Nobutaka Nakashima, Akita, H., Hoshino, T.. Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin, Metabolic Engineering, Vol. 25, pp. 204-214, 2014.  公式リンク  
  
• Nobutaka Nakashima, Miyazaki, K.. Bacterial cellular engineering by genome editing and gene silencing, International Journal of Molecular Sciences, Vol. 15, No. 2, pp. 2773-2793, 2014.  公式リンク  
  
• Nobutaka Nakashima, Tamura, T.. Gene silencing in Escherichia coli using antisense RNAs expressed from doxycycline-inducible vectors, Letters in Applied Microbiology, Vol. 56, No. 6, pp. 436-442, 2013.  公式リンク  
  
• Nobutaka Nakashima. Subtracting gene function by gene silencing and disruption in bacteria., Jan. 2012.
• Kagawa, Y., Mitani, Y., Yun, H.-Y., Nobutaka Nakashima, Tamura, N., Tamura, T.. Identification of a methanol-inducible promoter from Rhodococcus erythropolis PR4 and its use as an expression vector, Journal of Bioscience and Bioengineering, Vol. 113, No. 5, pp. 596-603, 2012.  公式リンク  
  
• Nobutaka Nakashima, Tamura, T.. A new carbon catabolite repression mutation of Escherichia coli, mlc, and its use for producing isobutanol, Journal of Bioscience and Bioengineering, Vol. 114, No. 1, pp. 38-44, 2012.  公式リンク  
  
• Nobutaka Nakashima, Tamura, T.. Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli, Nucleic Acids Research, Vol. 37, No. 15, 2009.  公式リンク  
  
• Goh, S., Boberek, J.M., Nobutaka Nakashima, Stach, J., Good, L.. Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli, PLoS ONE, Vol. 4, No. 6, 2009.  公式リンク  
  
• Nobutaka Nakashima. Advances in the development of genetic tools for the genus Rhodococcus, Jan. 2006.
• Kimura, N., Kitagawa, W., Mori, T., Nobutaka Nakashima, Tamura, T., Kamagata, Y.. Genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from Rhodococcus opacus strain SAO101, Applied Microbiology and Biotechnology, Vol. 73, No. 3, pp. 474-484, 2006.  公式リンク  
  
• Nobutaka Nakashima, Tamura, T., Good, L.. Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli, Nucleic Acids Research, Vol. 34, No. 20, 2006.  公式リンク  
  
• Todaka, Y., Wang, Y., Tashiro, K., Nobutaka Nakashima, Nishimoto, T., Sekiguchi, T.. Association of the GTP-binding protein Gtr1p with Rpc19p, a shared subunit of RNA polymerase I and III in yeast Saccharomyces cerevisiae, Genetics, Vol. 170, No. 4, pp. 1515-1524, 2005.  公式リンク  
  
• Nobutaka Nakashima, Mitani, Y., Tamura, T.. Actinomycetes as host cells for production of recombinant proteins, Microbial Cell Factories, Vol. 4, 2005.  公式リンク  
  
• Wang, Y., Nobutaka Nakashima, Sekiguchi, T., Nishimoto, T.. Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p, Biochemical and Biophysical Research Communications, Vol. 336, No. 2, pp. 639-645, 2005.  公式リンク  
  
• Nobutaka Nakashima, Tamura, T.. Isolation and characterization of a rolling-circle-type plasmid from Rhodococcus erythropolis and application of the plasmid to multiple-recombinant- protein expression, Applied and Environmental Microbiology, Vol. 70, No. 9, pp. 5557-5568, 2004.  公式リンク  
  
• Nobutaka Nakashima, Tamura, T.. Cell-free protein synthesis using cell extract of Pseudomonas fluorescens and CspA promoter, Biochemical and Biophysical Research Communications, Vol. 319, No. 2, pp. 671-676, 2004.  公式リンク  
  
• Nobutaka Nakashima, Tamura, T.. A Novel System for Expressing Recombinant Proteins over a Wide Temperature Range from 4 to 35°C, Biotechnology and Bioengineering, Vol. 86, No. 2, pp. 136-148, 2004.  公式リンク  
  
• Sekiguchi, T., Todaka, Y., Wang, Y., Hirose, E., Nobutaka Nakashima, Nishimoto, T.. A Novel Human Nucleolar Protein, Nop132, Binds to the G Proteins, RRAG A/C/D, Journal of Biological Chemistry, Vol. 279, No. 9, pp. 8343-8350, 2004.  公式リンク  
  
• Dong, L., Nobutaka Nakashima, Tamura, N., Tamura, T.. Isolation and characterization of the Rhodococcus opacus thiostrepton-inducible genes tipAL and tipAS: Application for recombinant protein expression in Rhodococcus, FEMS Microbiology Letters, Vol. 237, No. 1, pp. 35-40, 2004.  公式リンク  
  
• Sekiguchi, T., Hirose, E., Nobutaka Nakashima, Ii, M., Nishimoto, T.. Novel G Proteins, Rag C and Rag D, Interact with GTP-binding Proteins, Rag A and Rag B, Journal of Biological Chemistry, Vol. 276, No. 10, pp. 7246-7257, 2001.  公式リンク  
  
• Suzuki, N., Noguchi, E., Nobutaka Nakashima, Oki, M., Ohba, T., Tartakoff, A., Ohishi, M., Nishimoto, T.. The Saccharomyces cerevisiae small GTPase, gsp1p/Ran, is involved in 3′ processing of 7S-to-5.8S rRNA and in degradation of the excised 5′-A0 fragment of 35S pre-rRNA, both of which are carried out by the exosome, Genetics, Vol. 158, No. 2, pp. 613-625, 2001.  公式リンク  
  
• Nobutaka Nakashima, Noguchi, E., Nishimoto, T.. Saccharomyces cerevisiae putative G protein, Gtr1p, which forms complexes with itself and a novel protein designated as Gtr2p, negatively regulates the Ran/Gsp1p G protein cycle through Gtr2p, Genetics, Vol. 152, No. 3, pp. 853-867, 1999.  公式リンク  
  
• Shiomi, T., Fukushima, K., Suzuki, N., Nobutaka Nakashima, Noguchi, E., Nishimoto, T.. Human Dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3, Journal of Biochemistry, Vol. 123, No. 5, pp. 883-890, 1998.  公式リンク  
  
• Shiomi, T., Fukushima, K., Suzuki, N., Nobutaka Nakashima, Noguchi, E., Nishimoto, T.. Correction: Human Dis3p, which binds to either GTP- or GDP-Ran, complements saccharomyces cerevisiae dis3 (Journal of Biochemistry (1998) 123, 5 (883-890)), Journal of Biochemistry, Vol. 124, No. 1, 1998.  公式リンク  
  
• Hirose, E., Nobutaka Nakashima, Sekiguchi, T., Nishimoto, T.. RagA is a functional homologue of S. cerevisiae Gtr1p involved in the Ran/Gsp1-GTPase pathway, Journal of Cell Science, Vol. 111, No. 1, pp. 11-21, 1998.  公式リンク  
  
• Noguchi, E., Hayashi, N., Nobutaka Nakashima, Nishimoto, T.. Yrb2p, a Nup2p-related yeast protein, has a functional overlap with Rna1p, a yeast Ran-GTPase-activating protein, Molecular and Cellular Biology, Vol. 17, No. 4, pp. 2235-2246, 1997.  公式リンク  
  
• Noguchi, E., Hayashi, N., Azuma, Y., Seki, T., Nakamura, M., Nobutaka Nakashima, Yanagida, M., He, X., Mueller, U., Sazer, S., Nishimoto, T.. Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1, EMBO Journal, Vol. 15, No. 20, pp. 5595-5605, 1996.  公式リンク  
  
• Nobutaka Nakashima, Hayashi, N., Noguchi, E., Nishimoto, T.. Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae, Journal of Cell Science, Vol. 109, No. 9, pp. 2311-2318, 1996.  公式リンク  
  

著書

• Nobutaka Nakashima, Goh, S., Good, L., Tamura, T.. Multiple-Gene Silencing Using Antisense RNAs in Escherichia coli, Methods in Molecular Biology, Vol. 815, pp. 307-319, 2012.  公式リンク  

国際会議発表 (査読有り)

• Nobutaka Nakashima, Tamura, T, Good, L. Large-loop antisense RNA hairpins provide stabilized and highly efficient antisense regulators in Escherichia coli, Faseb Journal, Vol. 20, No. 4, pp. A538, 2006.  公式リンク  
  

[ BibTeX 形式で保存 ]    [ 論文・著書をCSV形式で保存 ]    [ 特許をCSV形式で保存 ]