論文

• Kaori Ando, Hisao Saneyoshi, Kohji Seio, Mitsuo Sekine. A theoretical study on the elimination reaction of acrylonitrile from 2′-O-cyanoethylated nucleosides by Bu4NF, Tetrahedron, Vol. 75, No. 1, 1-9, Jan. 2019.
• Takashi Kanamori, Masaki Y, Oda Y, Ohzeki H, Ohkubo A, Sekine M, Seio K.. DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl) deoxyuridine, Org. Biomol Chem., Jan. 2019.
• Yoshiaki Masaki, Keishi Yamamoto, Takeshi Inde, Keita Yoshida, Atsuya Maruyama, Tetsuya Nagata, Jun Tanihata, Shin'ichi Takeda, Mitsuo Sekine, Kohji Seio. Synthesis of 2'-O-(N-methylcarbamoylethyl) 5-methyl-2-thiouridine and its application to splice-switching oligonucleotides, Bioorganic & Medicinal Chemistry Letters, Dec. 2018.
• Yoshiaki Masaki, Yusuke Iriyama, Hiroyuki Nakajima, Yusuke Kuroda, Tatsuro Kanaki, Satoshi Furukawa, Mitsuo Sekine, Kohji Seio. Application of 2′-O-(2-N-Methylcarbamoylethyl) Nucleotides in RNase H-Dependent Antisense Oligonucleotides, Nucleic Acid Ther., Vol. 28, No. 5, Sept. 2018.
• Takeshi Inde, Shuhei Nishizawa, Yuusaku Hattori, Takashi Kanamori, Hideya Yuasa, Kohji Seio, Mitsuo Sekine, Akihiro Ohkubo. Synthesis of and triplex formation in oligonucleotides containing 2’-deoxy-6-thioxanthosine, July 2018.
• Takahito Tomori, Kento Nagaoka, Leo Takeshita, Takashi Shiozawa, Yuya Miyatake, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio. Deoxynucleoside Triphosphate Containing Pyridazin-3-one Aglycon as a Thymidine Triphosphate Substitute for Primer Extension and Chain Elongation by Klenow fragments, J. Org. Chem., Vol. 83, No. 15, pp. 8353–8363, June 2018.
• K. Ohno, D. Sugiyama, L. Takeshita, T. Kanamori, Y. Masaki, M. Sekine, K. Seio. Synthesis of photocaged 6-O-(2-nitrobenzyl) guanosine and 4-O-(2-nitrobenzyl)uridine triphosphates for photocontrol of the RNA transcription reaction, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 25, No. 21, pp. 6007-6015, Nov. 2017.
• T. Inde, Y. Masaki, A. Maruyama, Y. Ito, N. Makio, Y. Miyatake, T. Tomori, M. Sekine, K. Seio. Synthesis of oligonucleotides containing 2-N-heteroarylguanine residues and their effect on duplex/triplex stability, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 15, No. 39, pp. 8371-8383, Oct. 2017.
• Masaki Yoshiaki, Sekine Mitsuo, Seio Kohji. Deformability Calculation for Estimation of the Relative Stability of Chemically Modified RNA Duplexes., Current protocols in nucleic acid chemistry, Vol. 68, pp. 7.27.1-7.27.10, 2017.  公式リンク  
  
• Kohji Seio, Yurie Ohno, Kentaro Ohno, Leo Takeshita, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine. Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl]thymidine, Bioorganic & Medicinal Chemistry Letters, Vol. 26, 19, Page 4861-4863, Oct. 2016.
• Yoshiaki Masaki, Hugo Itou, Yuki Oda, Kazufumi Yamazaki, Nobuhiro Tago, Kentaro Ohno, Nozomi Ishii, Hirosuke Tsunoda, Takashi Kanamori, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio. Enzymatic synthesis and reverse transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate, Chemical Communications, Sept. 2016.
• Munefumi Tokugawa, Yoshiaki Masaki, Jan Christian Canggadibrata, Kazuhei Kaneko, Takashi Shiozawa, Takashi Kanamori, Morten Grøtli, L. Marcus Wilhelmsson, Mitsuo Sekine, Kohji Seio. 7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein, Chemical Communications, Vol. 52, 3809-3812, Sept. 2016.
• N. Tago, A. Katolik, N. E. Clark, E. J. Montemayor, K. Seio, M. Sekine, P. J. Hart, M. J. Damha. Design, synthesis, and properties of phosphoramidate 2 ',5 '-linked branched RNA: Toward the rational design of inhibitors of the RNA lariat debranching enzyme, OURNAL OF ORGANIC CHEMISTRY, Vol. 80, No. 20, pp. 10108-10118, Oct. 2015.
• Yoshiaki Masaki, Takeshi Inde, Tetsuya Nagata, Jun Tanihata, Takashi Kanamori, Kohji Seio, Shin'ichi Takeda, Mitsuo Sekine. Enhancement of exon skipping in mdx52 mice by 2′-O-methyl-2-thioribothymidine incorporation into phosphorothioate oligonucleotides, Med. Chem. Commun., Vol. 6, Page 630-633, Nov. 2014.
• Masaki Yoshiaki, Yamada Takeshi, Saneyoshi Hisao, Ohkubo Akihiro, Seio Kohji, Sekine Mitsuo. Synthesis and Exon-Skipping Activity of Chemically Modified RNAs, Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications, pp. 497-510, Mar. 2014.  公式リンク  
  
• Y. Masaki, R. Miyasaka, K. Hirai, T. Kanamori, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine. Properties of 5- and/or 2-modified 2 '-O-cyanoethyl uridine residue: 2 '-O-cyanoethyl-5-propynyl-2-thiouridine as an efficient duplex stabilizing component, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 12, No. 7, pp. 1157-1162, 2014.
• T. Yamada, Y. Masaki, N. Okaniwa, T. Kanamori, A. Ohkubo, H. Tsunoda, K. Seio, M. Sekine. Synthesis and properties of oligonucleotides modified with 2 '-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 12, No. 33, pp. 6457-6464, 2014.
• K. Yamada, Y. Masaki, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine. A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 12, No. 14, pp. 2255-2262, 2014.
• Y. Iijima, S. Kojima, E. Kodama, S. Kurohagi, T. Kanamori, Y. Masaki, A. Ohkubo, M. Sekine, K. Seio. Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3 '-terminus, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 11, No. 47, pp. 8276-8282, Dec. 2013.
• K. Seio, M. Tokugawa, H. Tsunoda, A. Ohkubo, F. Arisaka, M. Sekine. Assembly of pyrene-modified DNA/RNA duplexes incorporating a G-rich single strand region, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, Vol. 23, No. 24, pp. 6822-6824, Dec. 2013.
• A. Ohkubo, Y. Kondo, M. Suzuki, H. Kobayashi, T. Kanamori, Y. Masaki, K. Seio, K. Nagai, M. Sekine. Chemical synthesis of U1 snRNA derivatives, ORGANIC LETTERS, Vol. 15, No. 17, pp. 4386-4389, Sept. 2013.
• K. Yamada, A. Ohkubo, Y. Esaka, T. Kanamori, Y. Masaki, K. Seio, M. Sekine. Base recognition of gap sites in DNA-DNA and DNA-RNA duplexes by short oligonucleotides, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, Vol. 23, No. 11, pp. 3448-3451, June 2013.
• K. Seio, T. Kanamori, M. Tokugawa, H. Ohzeki, Y. Masaki, H. Tsunoda, A. Ohkubo, M. Sekine. Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 21, No. 11, pp. 3197-3201, June 2013.
• K. Yamada, Y. Hattori, T. Inde, T. Kanamori, A. Ohkubo, K. Seio, M. Sekine. Remarkable stabilization of antiparallel DNA triplexes by strong stacking effects of consecutively modified nucleobases containing thiocarbonyl groups, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, Vol. 23, No. 3, pp. 776-778, Feb. 2013.
• K. Seio, M. Tokugawa, T. Kanamori, H. Tsunoda, A. Ohkubo, M. Sekine. Synthesis and properties of cationic 2 '-O- N-(4-aminobutyl)carbamoyl modified oligonucleotides, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, Vol. 22, No. 7, pp. 2470-2473, Apr. 2012.
• T. Harakawa, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine. Development of an efficient method for phosphorodiamidate bond formation by using inorganic salts, Bioorganic & Medicinal Chemistry Letters, Vol. 22, No. 3, pp. 1445-1447, Feb. 2012.
• A. Ohkubo, N. Tago, A. Yokouchi, ; Y. Nishino, K. Yamada, H. Tsunoda, K. Seio, M. Sekine. Synthesis of 5 '-terminal capped oligonucleotides using O-N phosphoryl migration of phosphoramidite derivatives, ORGANIC LETTERS, Vol. 14, No. 1, pp. 10-13, Jan. 2012.
• Y. Masaki, R. Miyasaka, K. Hirai, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine. Prediction of the stability of modified RNA duplexes based on deformability analysis: Oligoribonucleotide derivatives modified with 2 '-o-cyanoethyl-5-propynyl-2-thiouridine as a promising component, CHEMICAL COMMUNICATIONS, Vol. 48, No. 58, pp. 7313-7315, 2012.
• T. Kanamori, Y. Masaki, M. Mizuta, H. Tsunoda, A. Ohkubo, M. Sekine, K. Seio. DNA duplexes and triplex-forming oligodeoxynucleotides incorporating modified nucleosides forming stable and selective triplexes, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 10, No. 5, pp. 1007-1013, 2012.
• K. Seio, S. Kurohagi, E. Kodama, Y. Masaki, H. Tsunoda, A. Ohkubo, M. Sekine. Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 10, No. 5, pp. 994-1006, 2012.
• A. Ohkubo, Y. Nishino, Y. Ito, H. Tsunoda, K. Seio, M. Sekine. Formation of new base pairs between inosine and 5-methyl-2-thiocytidine derivatives, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 10, No. 10, pp. 2008-2010, 2012.
• A. Ohkubo, A. Yokouchi, K. Yamada, H. Tsunoda, K. Seio, M. Sekine. Synthesis and properties of oligonucleotides having 5 '-terminal 2,2,7-trimethylguanosine cap analogues, NUCLEIC ACID THERAPEUTICS, Vol. 21, No. 5, pp. A39-A40, Oct. 2011.
• T. Yamada, N. Okaniwa, H. Saneyoshi, A. Ohkubo, K. Seio, T. Nagata, Y. Aoki, S. Takeda, M. Sekine. Synthesis of 2 '-O- 2-(N-methylcarbamoyl)ethyl ribonucleosides using oxa-michael reaction and chemical and biological properties of oligonucleotide derivatives incorporating these modified ribonucleosides, JOURNAL OF ORGANIC CHEMISTRY, Vol. 76, No. 9, pp. ç, May 2011.
• H. Tsunoda, T. Kudo, Y. Masaki, A. Ohkubo, K. Seio, M. Sekine. Biochemical behavior of N-oxidized cytosine and adenine bases in DNA polymerase-mediated primer extension reactions, NUCLEIC ACIDS RESEARCH, Vol. 39, No. 7, pp. 2995-3004, Apr. 2011.
• S. Q. Cao, I. Okamoto, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine. Synthesis and triplex-forming properties of oligonucleotides containing thio-substituted C-nucleoside 4-thiopseudoisocytidine, TETRAHEDRON LETTERS, Vol. 52, No. 3, pp. 407-410, Jan. 2011.
• M. Sekine, Y. Oeda, Y. Iijima, H. Taguchi, A. Ohkubo, K. Seio. Synthesis and hybridization properties of 2 '-O-methylated oligoribonucleotides incorporating 2 '-O-naphthyluridines, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 9, No. 1, pp. 210-218, 2011.
• A. Ohkubo, Y. Nishino, A. Yokouchi, Y. Ito, Y. Noma, Y. Kakishima, Y. Masaki, H. Tsunoda, K. Seio, M. Sekine. Stable triplex formation using the strong stacking effect of consecutive thionucleoside moieties, CHEMICAL COMMUNICATIONS, Vol. 47, No. 46, pp. 12556-12558, 2011.
• H. Tsunoda, T. Kudo, A. Ohkubo, K. Seio, M. Sekine. Synthesis of oligodeoxynucleotides using fully protected deoxynucleoside 3 '-phosphoramidite building blocks and base recognition of oligodeoxynucleotides incorporating N-3-cyano-ethylthymine, MOLECULES, Vol. 15, No. 11, pp. 7509-7531, Nov. 2010.
• K. Yamada, H. Taguchi, A. Ohkubo, K. Seio, M. Sekine. Synthesis and biochemical properties of oligodeoxynucleotides acylated by the chemically stable 2-(trimethylsilyl)benzoyl (TMSBz) group at the 5 ' or 3 ' terminus, Tetrahedron letters, Vol. 51, No. 39, pp. 5173-5176, Sept. 2010.
• A. Ohkubo, T. Sakaue, H. Tsunoda, K. Seio, M. Sekine. Synthesis and hybridization properties of oligonucleotides incorporating bi- and tricyclic cytosine derivatives, Chemistry letters, Vol. 39, No. 7, pp. 726-727, July 2010.
• A. Ohkubo, Y. Kuwayama, Y. Nishino, H. Tsunoda, K. Seio, M. Sekine. Oligonucleotide synthesis involving deprotection of amidine-type protecting groups for nucleobases under acidic conditions, ORGANIC LETTERS, Vol. 12, No. 11, pp. 2496-2499, June 2010.
• Y. Masaki, R. Miyasaka, A. Ohkubo, K. Seio, M. Sekine. Linear relationship between deformability and thermal stability of 2 '-O-modified RNA hetero duplexes, JOURNAL OF PHYSICAL CHEMISTRY B, Vol. 114, No. 7, pp. 2517-2524, Feb. 2010.
• Y. Masaki, A. Ohkubo, K. Seio, M. Sekine. Synthesis of 6-N-(benzothiazol-2-yl)deoxyadenosine and its exciton-coupled circular dichroism, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 18, No. 2, pp. 567-572, Jan. 2010.
• K. Seio, M. Shiraishi, E. Utagawa, A. Ohkubo, M. Sekine. Synthesis of oligodeoxynucleotides using the oxidatively cleavable 4-methoxytritylthio (MMTrS) group for protection of the 5 '-hydroxyl group, NEW JOURNAL OF CHEMISTRY, Vol. 34, No. 5, pp. 984-992, 2010.
• Y. Oeda, Y. Iijima, H. Taguchi, A. Ohkubo, K. Seio, M. Sekine. Microwave-Assisted Synthesis of 2 '-O-Aryluridine Derivatives, ORGANIC LETTERS, Vol. 11, No. 24, pp. 5582-5585, Dec. 2009.
• K. Seio, R. Tawarada, T. Sasami, M. Serizawa, M. Ise, A. Ohkubo, M. Sekine. Synthesis and hybridization of 2 '-O-methyl-RNAs incorporating 2 '-O-carbamoyluridine and unique participation of the carbamoyl group in U-G base pair, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 17, No. 20, pp. 7275-7280, Oct. 2009.
• Seio Kohji, Miyazaki Kazuya, Kurohagi Sayako, Masaki Yoshiaki, Tsunoda Hirosuke, Ohkubo Akihiro, Sekine Mitsuo. Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization., Nucleic acids symposium series (2004), No. 53, pp. 13-4, Sept. 2009.  公式リンク  
  
• K. Yamada, H. Taguchi, A. Ohkubo, K. Seio, M. Sekine. Synthesis and properties of nucleoside derivatives acylated by chemically stable 2-(trimethylsilyl)benzoyl group, Bioorganic & Medicinal Chemistry, Vol. 17, No. 16, pp. 5928-5932, Aug. 2009.
• A. Ohkubo, K. Sasaki, Y. Noma, H. Tsunoda, K. Seio, M. Sekine. Efficient solid-phase synthesis of oligodeoxynucleotides having a 5 '-terminal 2,2,7-trimethylguanosine pyrophosphate linkage, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 17, No. 13, pp. 4819-4824, July 2009.
• M. Mizuta, K. Seio, A. Ohkubo, M. Sekine. Fluorescence Properties of Pyrimidopyrimidoindole Nucleoside dC(PPI) Incorporated into Oligodeoxynucleotides, JOURNAL OF PHYSICAL CHEMISTRY B, Vol. 113, No. 28, pp. 9562-9569, July 2009.
• A. Ohkubo, Y. Noma, K. Aoki, H. Tsunoda, K. Seio, M. Sekine. Introduction of 3 '-Terminal Nucleosides Having a Silyl-Type Linker into Polymer Supports without Base Protection, JOURNAL OF ORGANIC CHEMISTRY, Vol. 74, No. 7, pp. 2817-2823, Apr. 2009.
• T. Sasami, R. Tawarada, A. Ohkubo, M. Sekine, K. Seio. Computational evaluation of the stability of 2 '-O-methyl-RNA/RNA duplexes incorporating 3-deazaguanine derivatives by ab initio calculations and a molecular dynamics simulation, JOURNAL OF MOLECULAR STRUCTURE-THEOCHEM, Vol. 899, No. 1-3, pp. 54-60, Apr. 2009.
• T. Sasami, Y. Odawara, A. Ohkubo, M. Sekine, K. Seio. Synthesis of oligodeoxynucleotides incorporating 2-N-carbamoylguanine and evaluation of the hybridization properties, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 17, No. 3, pp. 1398-1403, Feb. 2009.
• I. Okamoto, S. Q. Cao, H. Tanaka, K. Seio, M. Sekine. Synthesis of 4-Thiopseudoisocytidine and 4-Thiopseudouridine as Components of Triplex-forming Oligonucleotides, CHEMISTRY LETTERS, Vol. 38, No. 2, pp. 174-175, Feb. 2009.
• K. Miyata, R. Tamamushi, H. Tsunoda, A. Ohkubo, K. Seio, M. Sekine. Synthesis and Triplex Formation of Oligonucleotides Containing 8-Thioxodeoxyadenosine, Organic Letters, Vol. 11, No. 3, pp. 605-608, Feb. 2009.
• K. Seio, Y. Takaku, K. Miyazaki, S. Kurohagi, Y. Masaki, A. Ohkubo, M. Sekine. Synthesis of terminally modified oligonucleotides and their hybridization dependence on the size of the target RNAs, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 7, No. 11, pp. 2440-2451, 2009.
• A. Ohkubo, R. Kasuya, K. Miyata, H. Tsunoda, K. Seio, M. Sekine. New thermolytic carbamoyl groups for the protection of nucleobases, ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 7, No. 4, pp. 687-694, 2009.
• R. Tawarada, K. Seio, M. Sekine. Mechanistic studies on oxidative condensation of a thymidine 3 '-H-phosphonate derivative with 3 '-O-acetylthymidine, Arkivoc, Vol. 3, pp. 264-273, 2009.
• K. Seio, M. Mizuta, K. Tasaki, K. Tamaki, A. Ohkubo, M. Sekine. Hybridization-dependent fluorescence of oligodeoxynucleotides incorporating new pyrene-modified adenosine residues, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 16, No. 17, pp. 8287-8293, Sept. 2008.
• M. Mizuta, J. I. Banba, T. Kanamori, R. Tawarada, A. Ohkubo, M. Sekine, K. Seio. New nucleotide pairs for stable DNA triplexes stabilized by stacking interaction, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 130, pp. 9622-+, July 2008.
• A. Ohkubo, Y. Kuwayama, T. Kudo, H. Tsunoda, K. Seio, M. Sekine. O-selective condensation using P-N bond cleavage in RNA synthesis without base protection, Organic letters, Vol. 10, No. 13, pp. 2793-2796, July 2008.
• I. Okamoto, K. Seio, M. Sekine. Study of the base discrimination ability of DNA and 2 '-O-methylated RNA oligomers containing 2-thiouracil bases towards complementary RNA or DNA strands and their application to single base mismatch detection, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 16, No. 11, pp. 6034-6041, June 2008.
• A. Ohkubo, R. Kasuya, K. Aoki, A. Kobori, H. Taguchi, K. Seio, M. Sekine. Efficient synthesis of functionalized oligodeoxyribonucleotides with base-labile groups using a new silyl linker, BIOORGANIC & MEDICINAL CHEMISTRY, Vol. 16, No. 9, pp. 5345-5351, May 2008.
• H. Saneyoshi, K. Tamaki, A. Ohkubo, K. Seio, M. Sekine. Synthesis and hybridization properties of 2 '-O-(tetrazol-5-yl)ethyl-modified oligonucleotides, TETRAHEDRON, Vol. 64, No. 19, pp. 4370-4376, May 2008.
• K. Taira, S. Nakamura, K. Nakano, D. Maehara, K. Okamoto, S. Arimoto, D. Loakes, L. Worth, R. M. Schaaper, K. Seio, M. Sekine, K. Negishi, T. Negishi. Binding of MutS protein to oligonucleotides containing a methylated or an ethylated guanine residue, and correlation with mutation frequency, MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, Vol. 640, No. 1-2, pp. 107-112, Apr. 2008.
• A. Ohkubo, R. Kasuya, K. Sakamoto, K. Miyata, H. Taguchi, H. Nagasawa, T. Tsukahara, T. Watanobe, Y. Maki, K. Seio, M. Sekine. 'Protected DNA Probes' capable of strong hybridization without removal of base protecting groups, NUCLEIC ACIDS RESEARCH, Vol. 36, No. 6, pp. 1952-1964, Apr. 2008.
• H. Tsunoda, A. Ohkubo, H. Taguchi, K. Seio, M. Sekine. Synthesis and properties of DNA oligomers containing 2 '-deoxynucleoside N-oxide derivatives, JOURNAL OF ORGANIC CHEMISTRY, Vol. 73, No. 4, pp. 1217-1224, Feb. 2008.
• M. Sumino, A. Ohkubo, H. Taguchi, K. Seio, M. Sekine. Synthesis and properties of oligodeoxynucleotides containing 5-carboxy-2'-deoxycytidines, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, Vol. 18, No. 1, pp. 274-277, Jan. 2008.
• R. Tawarada, K. Seio, M. Sekine. Synthesis and properties of oligonucleotides with iodo-substituted aromatic aglycons: Investigation of possible halogen bonding base pairs, JOURNAL OF ORGANIC CHEMISTRY, Vol. 73, No. 2, pp. 383-390, Jan. 2008.
• A. Ohkubo, K. Tanaka, H. Taguchi, K. Seio, H. Nagasawa, T. Tsukahara, M. Sekine. An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, Vol. 17, No. 21, pp. 5969-5973, Nov. 2007.
• Saneyoshi Hisao, Okamoto Itaru, Masaki Yoshiaki, Ohkubo Akihiro, Seio Kohji, Mitsuo. Facile synthesis of 2 '-O-cyanoethyluridine by ring-opening reaction of 2,2 '-anhydrouridine with cyanoethyl trimethylsilyl ether in the presence of BF3 center dot Et2O, Tetrahedron Letters, Vol. 48, No. 48, pp. 8554-8557, Nov. 2007.  公式リンク  
  
• H. Saneyoshi, K. Ando, K. Seio, M. Sekine. Chemical synthesis of RNA via 2 '-O-cyanoethylated intermediates, TETRAHEDRON, Vol. 63, No. 45, pp. 11195-11203, Nov. 2007.
• E. Utagawa, A. Ohkubo, M. Sekine, K. Seio. Synthesis of branched Oligonucleotides with three different sequences using an oxidatively removable tritylthio group, JOURNAL OF ORGANIC CHEMISTRY, Vol. 72, No. 22, pp. 8259-8266, Oct. 2007.
• T. Sasami, Y. Odawara, A. Ohkubo, M. Sekine, K. Seio. Synthesis and hybridization properties of oligodeoxynucleotides incorporating 2-N-carbamoylguanine derivatives as guanine analogs, TETRAHEDRON LETTERS, Vol. 48, No. 30, pp. 5325-5329, July 2007.
• A. Ohkubo, H. Taguchi, K. Seio, H. Nagasawa, T. Tsukahara, M. Sekine. A new hydrophobic linker effective for the in situ synthesis of DNA-CPG conjugates as tools for SNP analysis, TETRAHEDRON LETTERS, Vol. 48, No. 29, pp. 5147-5150, July 2007.
• M. Mizuta, K. Seio, K. Miyata, M. Sekine. Fluorescent pyrimidopyrimidoindole nucleosides: Control of photophysical characterizations by substituent effects, JOURNAL OF ORGANIC CHEMISTRY, Vol. 72, No. 14, pp. 5046-5055, July 2007.
• B. Karwowski, K. Seio, M. Sekine. Chemical properties of 4,5-di(ethoxycarbonyl)-1,3-dioxolan-2-yl (DECDO) as a hydroxyl protecting group of the 2 '-hydroxyl function in ribonucleosides, JOURNAL OF HETEROCYCLIC CHEMISTRY, Vol. 44, No. 2, pp. 329-336, Apr. 2007.
• K. Seio, T. Sasami, A. Ohkubo, K. Ando, M. Sekine. Highly selective recognition of cytosine over uracil and adenine by a guanine analogue, 2-N-Acetyl-3-deazaguanine, in 2 '-O-Methyl-RNA/RNA and DNA duplexes, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 129, No. 5, pp. 1026-1027, Feb. 2007.
• K. Miyata, R. Mineo, R. Tamamushi, M. Mizuta, A. Ohkubo, H. Taguchi, K. Seio, T. Santa, M. Sekine. Synthesis and fluorescent properties of bi- and tricyclic 4-N-carbamoyldeoxycytidine derivatives, JOURNAL OF ORGANIC CHEMISTRY, Vol. 72, No. 1, pp. 102-108, Jan. 2007.
• K. Seio, T. Terada, M. Mizuta, A. Ohkubo, H. Taguchi, M. Sekine. Synthesis and hybridization properties of oligodeoxynucleotides with long-chain linkers, HELVETICA CHIMICA ACTA, Vol. 90, No. 10, pp. 1946-1965, 2007.
• M. Mizuta, K. Seio, K. Miyata, A. Ohkubo, H. Taguchi, M. Sekine. A pyrimidopyrimidoindole nucleoside (dC(PPI)): Photophysical properties and thermal stability of the modified DNA duplexes, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, Vol. 26, No. 10-12, pp. 1335-1338, 2007.
• E. Utagawa, M. Sekine, K. Seio. cis-tetrahydrofuran-3,4-diol structure as a key skeleton of new protecting groups removable by self-cyclization under oxidative conditions, JOURNAL OF ORGANIC CHEMISTRY, Vol. 71, No. 20, pp. 7668-7677, Sept. 2006.
• K. Miyata, R. Tamamushi, A. Ohkubo, H. Taguchi, K. Seio, T. Santa, M. Sekine. Synthesis and properties of a new fluorescent bicyclic 4-N-carbamoyldeoxycytidine derivative, ORGANIC LETTERS, Vol. 8, No. 8, pp. 1545-1548, Apr. 2006.
• I. Okamoto, K. Shohda, K. Seio, M. Sekine. Incorporation of 2 '-O-methyl-2-thiouridine into oligoribonucleotides induced stable A-form structure, CHEMISTRY LETTERS, Vol. 35, No. 1, pp. 136-137, Jan. 2006.
• H. Taguchi, A. Ohkubo, M. Sekine, K. Seio, H. Kakeya, H. Osada, T. Sasaki. Synthesis and biological properties of new phosmidosine analogs having an N-acylsulfamate linkage, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, Vol. 25, No. 4-6, pp. 647-654, 2006.
• K. Seio, T. Miyashita, K. Sato, M. Sekine. Synthesis and properties of new nucleotide analogues possessing squaramide moieties as new phosphate isosters, EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, Vol. 24, pp. 5163-5170, Dec. 2005.
• M. Sekine, M. Aoyagi, M. Ushioda, A. Ohkubo, K. Seio. Chemically stabilized phenylboranylidene groups having a dimethoxytrityl group as a colorimetrically detectable protecting group designed for cis-1,2-diol functions of ribonucleosides in the solid-phase synthesis of m(2)(2,2)G5 ' ppT, JOURNAL OF ORGANIC CHEMISTRY, Vol. 70, No. 21, pp. 8400-8408, Oct. 2005.
• K. Seio, H. Ukawa, K. Shohda, M. Sekine. Computational evaluation of intermolecular interactions of a universal base 3-nitropyrrole in stacked dimers and DNA duplexes, JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, Vol. 22, No. 6, pp. 735-746, June 2005.
• K. Seio, T. Negishi, K. Negishi, M. Sekine. Mild and facile deprotection for the synthesis of oligodeoxynucleotide incorporating a 6-O-ethyl-deoxyguanosine, LETTERS IN ORGANIC CHEMISTRY, Vol. 2, No. 2, pp. 179-183, Mar. 2005.
• K. Seio, T. Sasaki, K. Yanagida, M. Baba, M. Sekine. Synthesis of benzodithiol-2-yl-substituted nucleoside derivatives as lead compounds having anti-bovine viral diarrhea virus activity, JOURNAL OF MEDICINAL CHEMISTRY, Vol. 47, No. 21, pp. 5265-5275, Oct. 2004.
• A. Ohkubo, Y. Ezawa, K. Seio, M. Sekine. O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 126, No. 35, pp. 10884-10896, Sept. 2004.
• K. Seio, E. Utagawa, M. Sekine. New protected protecting groups for the 5 '-hydroxy group of deoxynucleosides by use of 2-(hydroxymethyl)- and 2-[(methylamino)methyl]benzoyl skeletons and oxidatively cleavable tritylthio and (4-methoxytrityl)thio groups, HELVETICA CHIMICA ACTA, Vol. 87, No. 9, pp. 2318-2333, 2004.
• A. Kobori, K. Miyata, M. Ushioda, K. Seio, M. Sekine. A new silyl ether-type linker useful for the automated synthesis of oligonucleotides having base-labile protective groups, CHEMISTRY LETTERS, Vol. 1, pp. 16-17, Jan. 2002.

著書

• Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine. Effects of 2'-O-Modifications on RNA Duplex Stability, Synthesis of Therapeutic Oligonucleotides, Springer, Jan. 2018.
• Ohkubo Akihiro, Okamoto Itaru, Kasuya Rintaro, Sakamoto Kazushi, Sasami Takeshi, Odawara Yoko, Masaki Yoshiaki, Sakaue Toshiyuki, Nagasawa Hiroshi, Tsukahara Toshifumi, Seio Kohji, Sekine Mitsuo. Protected DNA probes (PDP): A new strategy for gene detection, Chemistry of Nucleic Acid Components, Vol. 10, pp. 94-102, 2008.  公式リンク  
• 清尾康志, 関根光雄. RNAの基礎知識, RNAi法とアンチセンス法： 関根光雄・多比良和誠 編, 講談社サイエンティフィク, 2005.

国際会議発表 (査読なし・不明)

• T. Inde, Y. Masaki, A. Maruyama, Y. Ito, N. Makio, Y. Miyatake, T. Tomori, M. Sekine, K. Seio. Synthesis of oligodeoxyribonucleotides containing 2-N-heteroarylguanine residues and their effect on higher-order structure stability, The 44th International Symposium on Nucleic Acids Chemistry & The 1st Annual Meeting of Japan Society of Nucleic Acids Chemistry, Nov. 2017.
• Yoshiaki Masaki, Mitsuo Sekine, KOHJI SEIO. Relationship between deformability of sugar-modified RNA duplexes and their melting temperatures, 13th Annual Meeting of the Oligonucleotide Therapeutics Society, Sept. 2017.
• Takeshi Inde, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio. Base Pair Recognition of Alpha-deoxyribonucleosides with Unmodified Nucleobase and 8-Oxo-adenine in Antiparallel DNA Triplex, The 43rd International Symposium on Nucleic Acids Chemistry, Sept. 2016.
• Takahito Tomori, Kento Nagaoka, Yuya Miyatake, Yoshiaki Masaki, Mitsuo Sekine, Kohji seio. Synthesis and Enzymatic Properties of C-nucleoside Triphosphate Containing Pyridazin-3-one As a Uracil Analog, The 43rd International Symposium on Nucleic Acids Chemistry, Sept. 2016.
• Leo Takeshita, Kentaro Ohno, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio. Chemical and Enzymatic Synthesis of Photo-caged DNA containing N1-Nitrobenzyl-2´-deoxypseudouridine for UV Dependent Regulation of Transcription, The 43rd International Symposium on Nucleic Acids Chemistry, Sept. 2016.
• Yoshiaki Masaki, Ryuta Miyasaka, Keishi Yamamoto, Keita Yoshida, Akihiro Ohkubo, Mitsuo Sekine, Kohji Seio. Deformability analysis of chemically modified RNA and its applicability for estimation of RNA duplex stability, The 43rd International Symposium on Nucleic Acids Chemistry, Sept. 2016.
• Yoshiaki Masaki, Keishi Yamamoto, Takeshi Inde, Tetsuya Nagata, Jun Tanihata, Yusuke Iriyama, Shin'ichi Takeda, Kohji Seio, Mitsuo Sekine. Enhancement of exon skipping by 2´-O-(N-methylcarbamoyl)ethyl (MCE) and/or nucleobase modified nucleosides incorporation into phosphorothioate oligonucleotides, 11th of Annual Meeting of the Oligonucleotide Therapeutics Society, Oct. 2015.
• Kentaro Ohno, Leo Takeshita, Yoshihiro Iijima, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio. Synthesis of oligonucleotides containing photo-caged 2´ -deoxypseudouridine for the regulation of DNA-protein interaction, The 42nd International Symposium on Nucleic Acids Chemistry, Sept. 2015.
• Ayano Yamazaki, Kei Yamaguchi, Takashi Kanamori, Yoshiaki Masaki, Mitsuo Sekine, Kohji Seio. Synthesis of oligodeoxynucleotides containing 2´-deoxy-pseudoisocytidine for the development of chemically modified DNA duplexes and triplexes, The 42nd International Symposium on Nucleic Acids Chemistry, Sept. 2015.
• Yu Ito, Takashi Kanamori, Yoshiaki Masaki, Akihiro Ohkubo, Kohji Seio, Mitsuo Sekine. Synthesis of deoxyuridine derivatives modified with 2,4-diaminopyrimidine moiety for the development of high affinity triplex forming oligonucleotides, The 42nd International Symposium on Nucleic Acids Chemistry 2015, Sept. 2015.

国内会議発表 (査読なし・不明)

• 印出健志, 正木慶昭, 宮武佑弥, 関根光雄, 清尾康志. α-デオキシヌクレオシドを含むオリゴヌクレオチドのアンチパラレル型三重鎖形成能と塩基識別能, 日本化学会第98回春季年会, Mar. 2018.
• 印出健志, 丸山敦也, 伊藤 優, 牧尾直明, 友利貴人, 正木慶昭, 関根光雄, 清尾康志. 2 位のアミノ基にヘテロアリール環を導入したデオキシグアノシン誘導体の合成と オリゴヌクレオチド中での性質評価, 第47回複素環化学討論会, Oct. 2017.
• 清尾康志, 正木慶昭, 山本恵士, 印出健志, 入山友輔, 中島宏之, 金木達朗, 黒田雄介, 古川賢, 谷端 淳, 永田哲也, 武田伸一, 関根 光雄. 2’-水酸基にカルバモイルエチル型修飾を有する人工核酸を用いたアンチセンス核酸の開発, 日本核酸医薬学会 第3回年会, July 2017.
• 正木 慶昭, 宮坂 隆太, 山本 恵士, 吉田 圭汰, 関根 光雄, 清尾 康志. 糖部修飾オリゴヌクレオチドの変形能解析と二重鎖融解温度の関係, 日本核酸医薬学会 第3回年会, July 2017.
• 正木 慶昭, 吉田 圭汰, 山本 恵士, 関根 光雄, 清尾 康志. 糖部化学修飾RNA二重鎖の変形能と二重鎖融解温度の関係, 第10回バイオ関連化学シンポジウム2016, Sept. 2016.
• 宮村 大地, 金森 功吏, 正木 慶昭, 関根 光雄, 清尾 康志. 塩基部5位に3-メチルベンゾフラン誘導体を導入した2'-デオキシウリジンの合成と蛍光特性, 日本化学会 第96春季年会, Mar. 2016.
• Hiroya Asami, Munefumi Tokugawa, Yoshiaki Masaki, Eric Gloaguen, Kohji Seio, Mitsuo Sekine, Michel Mons. An Effective Molecular Model for Nanosecond Laser Spectroscopy of Short-lived Nucleobase: Application to Anharmonic Vibrational Analysis in the Mid-IR Region, 日本化学会 第96春季年会, Mar. 2016.
• 小田雄貴, 伊藤比佑吾, 金森功吏, 大窪章寛, 正木慶昭, 関根光雄, 清尾康志. 塩基性条件下不安定なヌクレオシド三リン酸の新規合成法の開発, 日本化学会 第96春季年会, Mar. 2016.
• 岸村智太, 印出健志, 正木慶昭, 関根光雄, 清尾康志. アンチセンス核酸の5´末端に糖を修飾するためのリンカーの設計と合成, 日本化学会 第96春季年会, Mar. 2016.
• 北川 諒, 田胡 信広, 関谷 彰太, 正木 慶昭, 関根 光雄, 清尾 康志. スルホンアミド骨格を有するヌクレオシド誘導体の合成と性質, 日本化学会 第96春季年会, Mar. 2016.
• 山本 恵士, 正木 慶昭, 吉田圭汰, 入山 友輔, 中嶋 宏之, 金木 達朗, 関根 光雄, 清尾 康志. 2-(N-エチルカルバモイル）エチル構造を有する糖部修飾核酸の合成と性質, 日本化学会 第96春季年会, Mar. 2016.
• 友利 貴人, 長岡 健斗, 宮武 佑弥, 正木 慶昭, 関根 光雄, 清尾 康志. ピリダジン-3-オンを核酸塩基部に有する/C/ヌクレオシドの合成と性質, 日本化学会 第96春季年会, Mar. 2016.
• 竹下 玲央, 大野 健太郎, 正木 慶昭, 関根 光雄, 清尾 康志. DNA-タンパク質相互作用の光制御をめざした光ケージされたデオキシシュードウリジンを含む核酸の合成および性質, 日本化学会 第96春季年会, Mar. 2016.
• Yoshiaki Masaki, Keishi Yamamoto, Takeshi Inde, Tetsuya Nagata, Jun Tanihata, Yusuke Iriyama, Shinishi Takeda, Mitsuo Sekine, KOHJI SEIO. Enhancement of exon skipping efficiency by using antisense oligonucleotides containing 2-thiothymidine derivatives, 日本核酸医薬学会第一回年会, Dec. 2015.
• 田胡信広, 北川諒, Adam Kotolik, Nathaniel E. Clark, Eric J. Montemayor, P. John Hart, 正木 慶昭, 金森 功吏, 関根 光雄, 清尾 康志, Masad J. Damha. 5'-アミノグアノシンを分岐部位に導入した2'-5'修飾分岐RNAの合成と性質, 第17回日本RNA学会年会, July 2015.
• 山本 恵士, 正木 慶昭, 石井 陽大, 印出 健志, 金森 功吏, 関根 光雄, 清尾 康志. 2'-O-メチルカルバモイルエチル修飾2-チオリボチミジンを含むオリゴヌクレオチドの合成と性質, 第17回日本RNA学会年会, July 2015.
• 大野 健太郎, 正木 慶昭, 金森 功吏, 関根 光雄, 清尾 康志. 光ケージヌクレオシド三リン酸の合成と性質, 第17回日本RNA学会年会, July 2015.

その他の論文・著書など

• 清尾康志, 岡本到, 関根光雄. 2-チオウリジンを利用したアンチセンス核酸の化学合成と性質, 生物工学会誌, Vol. 82, pp. 139-141, 2004.

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