論文

• Takayuki Miki, Masahiro Hashimoto, Hiroki Takahashi, Masatoshi Shimizu, Sae Nakayama, Tadaomi Furuta, Hisakazu Mihara. De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cells, Nat. Commun., vol. 15, pp. 8503, Oct. 2024.
• Takayuki Miki, Keigo Kajiwara, Sae Nakayama, Masahiro Hashimoto, Hisakazu Mihara. Effects of Hydrophobic Residues on the Intracellular Self-Assembly of De Novo Designed Peptide Tags and Their Orthogonality, ACS Synthetic Biology, Mar. 2022.  公式リンク  
  
• Takayuki Miki, Masahiro Hashimoto, Taichi Nakai, Hisakazu Mihara. A guide-tag system controlling client enrichment into Y15 peptide-based granules for an in-cell protein recruitment assay, Chemical Communications, Royal Society of Chemistry, Vol. 57, p. 11338, Oct. 2021.
• Jyh Yea Chia, Takayuki Miki, Hisakazu Mihara, Hiroshi Tsutsumi. Biofunctional supramolecular hydrogels fabricated from a short self-assembling peptide modified with bioactive sequences for the 3D culture of breast cancer MCF-7 cells, Bioorganic & Medicinal Chemistry, Vol. 46, pp. 116345, Sept. 2021.  公式リンク  
  
• Takayuki Miki, Taichi Nakai, Masahiro Hashimoto, Keigo Kajiwara, Hiroshi Tsutsumi, Hisakazu Mihara. Intracellular artificial supramolecules based on de novo designed Y15 peptides, Nature Communications, Vol. 12, June 2021.  公式リンク  
  
• Masahiro Hashimoto, Takayuki Miki, Iou Ven Chang, Hiroshi Tsutsumi, Hisakazu Mihara. Selection of fluorescent biosensors against galectin-3 from an NBD-modified phage library displaying designed α-helical peptides, Bioorganic & Medicinal Chemistry Letters, 37, 127835, Feb. 2021.
• Teerapat Anananuchatkul, Hiroshi Tsutsumi, Takayuki Miki, Hisakazu Mihara.. hDM2 Protein-Binding Peptides Screened from Stapled alpha-Helical Peptide Phage Display Libraries with Different Types of Staple Linkers., Bioorganic & Medicinal Chemistry Letters, Vol. 30, 127605, Nov. 2020.
• Teerapat Anananuchatkul, Iou Ven Chang, Takayuki Miki, Hiroshi Tsutsumi, Hisakazu Mihara. Construction of a Stapled α‑Helix Peptide Library Displayed on Phage for the Screening of Galectin-3-Binding Peptide Ligands, ACS Omega, ACS Publications, Vol. 5, p. 5666-5674, Mar. 2020.
• Yuki Nishikawa, Takayuki Miki, Masashi Awa, Keiko Kuwata, Tomonori Tamura, Itaru Hamachi. Development of a Nitric Oxide-Responsive Labeling Reagent for Proteome Analysis of Live Cells, ACS Chemical Biology, Vol. 14, p. 397, Feb. 2019.
• Sarah Hostachy, Marie Masuda, Takayuki Miki, Itaru Hamachi, Sandrine Sagan, Olivier Lequin, Kadda Medjoubi, Andrea Somogyi, Nicolas Delsuc, Clotilde Policar. Graftable SCoMPIs enable the labeling and X-ray fluorescence imaging of proteins, Chemical Science, Vol. 9, No. 19, pp. 4483-4487, 2018.  公式リンク  
  
• Takayuki Miki, Masashi Awa, Yuki Nishikawa, Shigeki Kiyonaka, Masaki Wakabayashi, Yasushi Ishihama, Itaru Hamachi. A conditional proteomics approach to identify proteins involved in zinc homeostasis, Nature Methods, Vol. 13, No. 11, pp. 931-937, 2016.  公式リンク  
  
• Zhining Song, Yousuke Takaoka, Yoshiyuki Kioi, Kazuhiro Komatsu, Tomonori Tamura, Takayuki Miki, Itaru Hamachi. Extended affinity-guided DMAP chemistry with a finely tuned acyl donor for intracellular FKBP12 labeling, Chemistry Letters, Vol. 44, No. 3, pp. 333-335, 2015.  公式リンク  
  
• Takayuki Miki, Sho-hei Fujishima, Kazuhiro Komatsu, Keiko Kuwata, Shigeki Kiyonaka, Itaru Hamachi. LDAI-based chemical labeling of intact membrane proteins and its pulse-chase analysis under live cell conditions, Chemistry and Biology, Vol. 21, No. 8, pp. 1013-1022, 2014.  公式リンク  
  
• Tomonori Tamura, Yoshiyuki Kioi, Takayuki Miki, Shinya Tsukiji, Itaru Hamachi. Fluorophore labeling of native FKBP12 by ligand-directed tosyl chemistry allows detection of its molecular interactions in vitro and in living cells, Journal of the American Chemical Society, Vol. 135, No. 18, pp. 6782-6785, 2013.  公式リンク  
  
• Kazuya Matsuo, Yoshiyuki Kioi, Ryosuke Yasui, Yousuke Takaoka, Takayuki Miki, Sho-hei Fujishima, Itaru Hamachi. One-step construction of caged carbonic anhydrase i using a ligand-directed acyl imidazole-based protein labeling method, Chemical Science, Vol. 4, No. 6, pp. 2573-2580, 2013.  公式リンク  
  
• Sho-hei Fujishima, Ryosuke Yasui, Takayuki Miki, Akio Ojida, Itaru Hamachi. Ligand-directed acyl imidazole chemistry for labeling of membrane-bound proteins on live cells, Journal of the American Chemical Society, Vol. 134, No. 9, pp. 3961-3964, 2012.  公式リンク  
  

著書

• 三木卓幸, 橋本匡浩, 三原久和. 細胞内でタンパク質を集積させるペプチドタグの開発, バイオサイエンスとインダストリー, Vol. 80, No. 2, pp. 120-121, Mar. 2022.
• 三木卓幸, 三原久和. 自己組織化ペプチドの設計と利用, 相分離生物学の全貌, 東京化学同人, Nov. 2020.

国際会議発表 (査読有り)

• Takayuki Miki. Development of β-sheet Y15 peptide for integrating protein in cells, Pacifichem 2021 Congress, Dec. 2021.

国際会議発表 (査読なし・不明)

• Takayuki Miki. Development of self-assembling peptidet tag for intracellular reconstitution of prorein assembly, 2022 Annual Spring Meeting of the Polymer Society of Korea, Apr. 2022.
• 三木卓幸. Progression of zero programming skilled scientist to python user, Scipy Japan Conference 2019, Apr. 2019.

国内会議発表 (査読なし・不明)

• Masahiro Hashimoto, Takayuki Miki, Hiroki Takahashi, Sae Nakayama, Tadaomi Furuta, Hisakazu Mihara. Intracellular bottom-up reconstruction of liquid droplet by fusion of YK amphiphilic peptide tag, The 60th Japanese Peptide Symposium, Nov. 2023.
• 橋本 匡浩, 三木 卓幸, 中井 太一, 丹羽 達也, 三原 久和. 細胞内で自己集合するペプチドタグ開発１：クラスター形成を伴う蛋白質間相互作用解析, Mar. 2022.
• 三木 卓幸, 高橋 広樹, 橋本 匡浩, 梶原 圭悟, 中山 彩恵, 三原 久和. 細胞内で自己集合するペプチドタグ開発２：蛋白質高次構造体の設計と構築, 日本化学会第１０２春季年会, Mar. 2022.
• Hiroki takahashi, Takayuki Miki, Taichi Nakai, Masahiro Hashimoto, Hisakazu Mihara. DEVELOPMENT OF AMPHIPHILIC YK PEPTIDE TAGS FOR BOTTOM-UP CONSTRUCTION OF LIQUID-LIQUID PHASE SEPARATION, The 58th Japanese Peptide Symposium, Oct. 2021.
• Takayuki Miki, Masahiro Hashimoto, Taich Nakai, Keigo Kajiwara, Hisakazu Mihara. Intracellular reconstitution and analysis of protein complexes by Yn self-assembling peptide tag, The 58th Japanese Peptide Symposium, Oct. 2021.
• 橋本 匡浩, 三木 卓幸, 中井 太一, 三原 久和. Yn設計ペプチドによる細胞内でのクラスター形成を伴う蛋白質間相互作用解析, 第１５回バイオ関連化学シンポジウム, Sept. 2021.
• 三木 卓幸, 橋本 匡浩, 高橋 広樹, 梶原 圭悟, 中山 彩恵, 中井 太一, 三原 久和. 両親媒性ペプチドによる細胞内での蛋白質高次構造体の形成, 第１５回バイオ関連化学シンポジウム, Sept. 2021.
• 筧 翔太, 瀬古 健太, 三木 卓幸, 三原 久和. Hisタグ融合hDM2を標的としたNi(II)錯体修飾ファージディスプレイ法の確立, 第１５回バイオ関連化学シンポジウム, Sept. 2021.

その他の論文・著書など

• 三木卓幸, 浜地格. 分子夾雑の合成化学を基軸としたケミカルプロテオミクス, 化学と工業, May 2019.
• 浜地格, 三木卓幸, 高岡洋輔. 高選択的in vivoタンパク質ラベル化による新イメージング技術, がんの分子イメージング, 化学同人, 216–225, 2015.

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